Comparison List


YFP3 is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2005, derived from Aequorea victoria. It has moderate acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
? Aequorea victoria 27.0 kDa -

FPbase ID: UPG56


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
514 527 99,000 0.76 75.24 5.79    


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YFP3 Sequence

YFP3 was derived from EYFP-F46L with the following mutations: V1adel/I47L/Q80R/S208F/V224L/L231E/D234N
amino acid numbers relative to avGFP. show relative to EYFP-F46L



YFP3 possessed six mutations that resulted in an RRC almost fivefold greater than that of the parental pair. YFP3 also conferred a roughly threefold improvement in FRET relative to the fast-maturing Venus YFP.

Nguyen & Daugherty (2005)

Primary Reference

Evolutionary optimization of fluorescent proteins for intracellular FRET

Nguyen Aw, Daugherty Ps

(2005). Nature Biotechnology, 23(3) , 355-360. doi: 10.1038/nbt1066. Article   Pubmed

Additional References

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