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|?||Aequorea victoria||27.0 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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YFP3 was derived from EYFP-F46L with the following mutations: V1adel/I47L/Q80R/S208F/V224L/L231E/D234N
amino acid numbers relative to avGFP. show relative to EYFP-F46L
YFP3 possessed six mutations that resulted in an RRC almost fivefold greater than that of the parental pair. YFP3 also conferred a roughly threefold improvement in FRET relative to the fast-maturing Venus YFP.
Nguyen & Daugherty (2005)
(2005). Nature Biotechnology, 23(3) , 355-360. doi: 10.1038/nbt1066. Article Pubmed
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