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Comparison List

Superfolder GFP

a.k.a. sfGFP

Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria.
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Oligomerization Organism Molecular Weight Cofactor
Weak dimer Aequorea victoria 26.8 kDa -

FPbase ID: B4SOW

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
485 510 83,300 0.65 54.15      
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Superfolder GFP OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
- 2.2 ± 0.1 (74 cells) U-2 OS Costantini et al. (2012)
62.4 ± 6.3 (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

No photostability measurements available ... add one!

Superfolder GFP Sequence

BLAST

Superfolder GFP was derived from Folding Reporter GFP with the following mutations: S30R/Y39N/N105T/Y145F/I171V/A206V

MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
GenBank: ASL68970
IPG: 154375264

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for Superfolder GFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Engineering and characterization of a superfolder green fluorescent protein

Pédelacq J-D, Cabantous S, Tran T, Terwilliger Tc, Waldo Gs

(2005). Nature Biotechnology, 24(1) , 79-88. doi: 10.1038/nbt1172. Article   Pubmed

Additional References

  1. A Novel Ultra-Stable, Monomeric Green Fluorescent Protein For Direct Volumetric Imaging of Whole Organs Using CLARITY

    Scott Dj, Gunn Nj, Yong Kj, Wimmer Vc, Veldhuis Na, Challis Lm, Haidar M, Petrou S, Bathgate Rad, Griffin Mdw

    (2018). Scientific Reports, 8(1) , 667. doi: 10.1038/s41598-017-18045-y. Article   Pubmed

  2. Synthetic Biology of Proteins: Tuning GFPs Folding and Stability with Fluoroproline

    Steiner T, Hess P, Bae Jh, Wiltschi B, Moroder L, Budisa N

    (2008). PLoS ONE, 3(2) , e1680. doi: 10.1371/journal.pone.0001680. Article   Pubmed

  3. A Comparison of the Fluorescence Dynamics of Single Molecules of a Green Fluorescent Protein: One- versus Two-Photon Excitation

    Cotlet M, Goodwin Pm, Waldo Gs, Werner Jh

    (2006). ChemPhysChem, 7(1) , 250-260. doi: 10.1002/cphc.200500247. Article   Pubmed

External Resources

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