Comparison List

Superfolder CFP

a.k.a. sfCFP

Superfolder CFP is a basic (constitutively fluorescent) cyan fluorescent protein published in 2005, derived from Aequorea victoria.

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Oligomerization Organism Molecular Weight Cofactor
Weak dimer Aequorea victoria 26.8 kDa -

FPbase ID: 41E1T


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
430 479          


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Superfolder CFP Sequence

Superfolder CFP was derived from Superfolder GFP with the following mutations: Y66W



We tested whether the superfolder mutations would improve the folding of color variants of folding reporter GFP. Blue, cyan and yellow fluorescent proteins (BFP, CFP, YFP) were produced from folding reporter GFP and superfolder GFP by incorporating the additional mutations Y66H (BFP), Y66W (CFP) and T203Y (YFP), respectively. E. coli colonies expressing the color variants of superfolder GFP as C-terminal fusions with poorly folded ferritin were brightly fluorescent…

Pédelacq et al. (2005)

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