|Monomer||Aequorea victoria||27.0 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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SHardonnay was derived from EYFP with the following mutations: Y203F
amino acid numbers relative to avGFP. show relative to EYFP
Our hypothesis also inspired the generation of an eYFP mutant, eliminating the centrosymmetry in the eYFP chromophore by replacing Tyr203 with Phe203 through site-directed mutagenesis. The mutant was named SHardonnay for its improved second-harmonic properties and for its color appearance similar to white wine of the Chardonnay grape.
De Meulenaere et al. (2013)
(2013). Journal of the American Chemical Society, 135(10) , 4061-4069. doi: 10.1021/ja400098b. Article Pubmed
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