(2013). Journal of the American Chemical Society, 135(10) , 4061-4069. doi: 10.1021/ja400098b. Article Pubmed
Our hypothesis also inspired the generation of an eYFP mutant, eliminating the centrosymmetry in the eYFP chromophore by replacing Tyr203 with Phe203 through site-directed mutagenesis. The mutant was named SHardonnay for its improved second-harmonic properties and for its color appearance similar to white wine of the Chardonnay grape.