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Comparison List

moxNeonGreen

moxNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2015, derived from Branchiostoma lanceolatum.

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Aggregation Organism Molecular Weight Cofactor
Monomer Branchiostoma lanceolatum 26.6 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
505 520 111,000 0.74 82.14      

Photostability

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moxNeonGreen Sequence

moxNeonGreen was derived from mNeonGreen with the following mutations: C139S
amino acid numbers relative to LanYFP. show relative to mNeonGreen

Note: Note: this sequence is a best guess. Constantini et al. (2015) says "we were able to create a cysteine-less green alternative to GFP family members, moxNeonGreen, an oxidizing environment-optimized variant of the recently described mNeonGreen", but does not provide more information. The sequence below is the result of mutating the only cysteine in mNeonGreen to serine.

MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWSRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK

Excerpts

We had different results with mNeonGreen and moxNeonGreen. Both versions formed actin stress fibers. However, neither version was significantly incorporated into microtubules. In the original report, it was unclear how long the linker was, but we tried both lengths and had the same result.

Costantini et al. (2015)

Primary Reference

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