Comparison List


miRFP670nano is a basic (constitutively fluorescent) near ir fluorescent protein published in 2019, derived from Nostoc punctiforme. It has very low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
Oligomerization Organism Molecular Weight Cofactor
Monomer Nostoc punctiforme 17.1 kDa Biliverdin

FPbase ID: 1KNNM


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
645 670 95,000 0.108 10.26 3.7 100.0  


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miRFP670nano Sequence

miRFP670nano was derived from NpR3784g with the following mutations: V7M/F25C/M26V/Y27F/P31E/S41A/A48S/N51K/Q55R/T57R/I72Y/G82N/H87Y/N99I/N117H/L136Q/Q139V/Q148_L149del

GenBank: MK176509


Deposited: ,


Unlike BphP-derived NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an internal protein tag.

Oliinyk et al. (2019)

miRFP670nano is an effective FRET donor for red-shifted NIR FPs, enabling engineering NIR FRET biosensors spectrally compatible with GFP-like FPs and blue–green optogenetic tools

Oliinyk et al. (2019)

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