| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Entacmaea quadricolor | 26.6 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 460 | 592 | 28,500 | 0.46 | 13.11 | 2.1 | 354.0 | 2.1 |
No photostability measurements available ... add one!
mCRISPRed was derived from mRuby3 with the following mutations: P142A/N143D/T158L/I160R/N186D
amino acid numbers relative to eqFP611. show relative to mRuby3
pH titration showed that mCRISPRed had the lowest pKa value (pKa = 2.1) among all fluorescent proteins known to date, surpassing that of TagBFP2 (pKa = 2.7), those of other comparable long Stokes shift red fluorescent proteins, and that of a green fluorescent protein variant named Gamillus engineered for increased pH resistance (pKa = 3.4). These properties render it very favorable for applications in acidic environments, such as lysosomes.
Erdogan et al. (2020)
(2020). Cell Chemical Biology, , . doi: 10.1016/j.chembiol.2020.02.004. Article Pubmed
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