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mCRISPRed

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Apr 13, 2020 19:04:13. Or view the full change history

mCRISPRed is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2020, derived from Entacmaea quadricolor. It has very low acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Entacmaea quadricolor	26.6 kDa	-

FPbase ID: RTMMX

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
460	592	28,500	0.46	13.11	2.1	354.0	2.1

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Photostability

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mCRISPRed Sequence

BLAST

mCRISPRed was derived from mRuby3 with the following mutations: P146A/N147D/T162L/I164R/N190DP142A/N143D/T158L/I160R/N186D
amino acid numbers relative to mRuby3eqFP611. show relative to eqFP611 show relative to mRuby3

MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEADTEMMYPADGGLRGYLDRALKVDGGGHLHCNFVTTYRSKKTVGDIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELYK

GenBank: MN844880.1

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for mCRISPRed
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells

Erdogan M, Fabritius A, Basquin J, Griesbeck O

(2020). Cell Chemical Biology, , . doi: 10.1016/j.chembiol.2020.02.004. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for mCRISPRed
Search RIKEN BRC for mCRISPRed

Change history

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