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Comparison List

mNeptune2

mNeptune2 is a basic (constitutively fluorescent) red fluorescent protein published in 2014, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with high acid sensitivity.

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Aggregation Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 27.4 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
599 651 89,000 0.24 21.36 6.3 27.0  

Photostability

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mNeptune2 Sequence

mNeptune2 was derived from mNeptune with the following mutations: A104V/I121L/I171H/K207N
amino acid numbers relative to eqFP578. show relative to mNeptune

MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGRIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK
GenBank: AHL19968
IPG: 49083568

Excerpts

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Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

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