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KillerRed was derived from anm2CP with the following mutations: V21E/D24G/I30V/K73R/N76D/T91S/G114D/I118V/K136R/T145N/S154P/C161A/Y162F/S175G/L181M/K190E/V199I/I201T/K205R/T220A/*233GextSDEDV21E/D24G/I30V/K73R/N76D/T91S/G114D/I118V/K136R/T145N/S154P/C161A/Y162F/S175G/L181M/K190E/V199I/I201T/K205R/T220A/*231GextSDED
We characterized KillerRed phototoxic activity as a function of irradiation time. KillerRed kills 96% of E. coli cells after 10 min and almost all cells after 20 min of irradiation with white light (1 W/cm2). We also verified that cell killing depends on the wavelength of the incident light. KillerRed demonstrated a strong cell killing effect in green light (540–580 nm) and almost no effect upon irradiation with blue light (460–490 nm) of equal intensity (35 mW/cm2).