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Comparison List

iRFP720

similar: miRFP720

iRFP720 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2013, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris 34.6 kDa Biliverdin

FPbase ID: OX75X

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
702 720 96,000 0.06 5.76 4.5    
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Photostability

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iRFP720 Sequence

BLAST

iRFP720 was derived from iRFP713 with the following mutations: E180S/T202F/V203I/V254N

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE
GenBank: AGN32866

Excerpts

No excerpts have been added for iRFP720
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Near-infrared fluorescent proteins for multicolor in vivo imaging

Shcherbakova Dm, Verkhusha Vv

(2013). Nature Methods, 10(8) , 751-754. doi: 10.1038/nmeth.2521. Article

Additional References

  1. Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720

    Fukuda A, Honda S, Fujioka N, Sekiguchi Y, Mizuno S, Miwa Y, Sugiyama F, Hayashi Y, Nishimura K, Hisatake K

    (2019). PLOS ONE, 14(11) , e0225213. doi: 10.1371/journal.pone.0225213. Article   Pubmed

External Resources

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