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Comparison List

emiRFP703

a.k.a. enhanced miRFP703

emiRFP703 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.2 kDa Biliverdin

FPbase ID: GNJK5

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
674 703 90,900 0.086 7.82 4.5    

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
700.0 200.0 (W) Arc-lamp Widefield 23.0 Matlashov et al. (2020)

emiRFP703 Sequence

emiRFP703 was derived from miRFP703 with the following mutations: M1_N19delinsMAEGSVARQPDLLT

MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES
GenBank: MN701053

Excerpts

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Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

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