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Comparison List

emiRFP703

a.k.a. enhanced miRFP703

emiRFP703 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.2 kDa Biliverdin

FPbase ID: GNJK5

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
674 703 90,900 0.086 7.82 4.5    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
700.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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emiRFP703 Sequence

BLAST

emiRFP703 was derived from miRFP703 with the following mutations: M1_N19delinsMAEGSVARQPDLLT

MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES
GenBank: MN701053

Excerpts

No excerpts have been added for emiRFP703
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article   Pubmed

Additional References

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