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Comparison List

emiRFP670

a.k.a. enhanced miRFP670

emiRFP670 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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emiRFP670 Spectrum Fluorescent protein emiRFP670 excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.2 kDa Biliverdin

FPbase ID: YHJV5

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
642 670 87,400 0.14 12.24 4.5   1.53
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
450.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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emiRFP670 Sequence

BLAST

emiRFP670 was derived from miRFP670 with the following mutations: M1_N19delinsMAEGSVARQPDLLT

MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES
GenBank: MN701051

Excerpts

No excerpts have been added for emiRFP670
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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