No spectrum has been submitted ... add a spectrum!
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Echinophyllia sp. SC22 | 25.4 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| On | 460 | 504 | 45,000 | 0.5 | 22.5 | |||
| Off |
No photostability measurements available ... add one!
bsDronpa was derived from rsFastLime with the following mutations: V60A/A69T/D112N/P141Q/G155S/M159C/F173C
During the screen for Padron*, we identified a mutant with a cysteine residue at position 159 that was negatively switchable but exhibited a broad asymmetric absorption spectrum that was shifted to shorter wavelengths. After further improvements, we obtained a bright green fluorescent Dronpa variant with eight mutations, displaying an absorption maximum at 460 nm in the on-state. Termed bsDronpa (for broad-absorption-spectrum Dronpa), this RSFP combines a relatively large Stokes shift of 44 nm with a broad on-state absorption spectrum. It is efficiently excited with UV light, while maintaining good switching characteristics.
Andresen et al. (2008)
(2008). Nature Biotechnology, 26(9) , 1035-1040. doi: 10.1038/nbt.1493. Article Pubmed
Something missing or incorrect? Submit a change Submit a change