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|State||Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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bsDronpa was derived from rsFastLime with the following mutations: V60A/A69T/D112N/P141Q/G155S/M159C/F173C
During the screen for Padron*, we identified a mutant with a cysteine residue at position 159 that was negatively switchable but exhibited a broad asymmetric absorption spectrum that was shifted to shorter wavelengths. After further improvements, we obtained a bright green fluorescent Dronpa variant with eight mutations, displaying an absorption maximum at 460 nm in the on-state. Termed bsDronpa (for broad-absorption-spectrum Dronpa), this RSFP combines a relatively large Stokes shift of 44 nm with a broad on-state absorption spectrum. It is efficiently excited with UV light, while maintaining good switching characteristics.