No spectrum has been submitted ... add a spectrum!
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Cytaeis uchidae | 25.6 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 496 | 504 |
No photostability measurements available ... add one!
QC2-6 was derived from (n1)oxStayGold with the following mutations: N132D/P151T/L155T/K162E
amino acid numbers relative to CU17S. show relative to (n1)oxStayGold
We comprehensively prepared several libraries of (n1)oxStayGold variants that carried different partial mutations at the interface. In one attempt, we focused on threonine substitution at Pro151 and Leu155 and screened candidates in a library of (n1)oxStayGold P151T/L155T iteratively with multiple cycles of random mutagenesis to produce QC2-6. This variant contained four mutations (P151T, L155T, N132D and K162E) relative to (n1)oxStayGold. When observing the fluorescence in the cytosolic and nuclear compartments of transfected cells under intense illumination, we confirmed that QC2-6 was highly photostable and bright.
Ando et al. (2023)
(2023). Nature Methods, , . doi: 10.1038/s41592-023-02085-6. Article Pubmed
Something missing or incorrect? Submit a change Submit a change