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Comparison List

Rosmarinus

Rosmarinus is a basic (constitutively fluorescent) cyan fluorescent protein published in 2017.

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Oligomerization Organism Molecular Weight Cofactor
Tetramer <add one> 26.1 kDa -

FPbase ID: GNU4X

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
437 482 45,000 0.85 38.25      

Photostability

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Rosmarinus Sequence

MSVIKSVMKIKLHMDGIVNGHKFMITGEGEGKPFEGTHTIILKVKEGGPLPFAYDILTTAFQYGNRVFTKYPKDIPDYFKQSFPEGYSWERSMTFEDQGVCTVTSDIKLEGDYFTYDIRFHGVNFPAGGPVMQKKTLRWEPSTENMYVRDGVLVGEVERTLLLEGNKHHRCNFRTTYKAKKEVVLPEYHFVDHRIEILGHDKDYNNVVVYENAVARQQASTLPSKAK
GenBank: KY931461

Excerpts

Rosmarinus remains tetrameric and becomes dimeric only at nanomolar concentrations (∼70 nM)... Rosmarinus was made using PCR and the In-Fusion cloning technique (Clontech) to fuse the front half of the coding region for mc5 to the back half of meleCFP at a five amino acid identical stretch in their coding sequences.

Molina et al. (2017)

Primary Reference

Additional References

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