Comparison List


AausFP1 is a basic (constitutively fluorescent) green fluorescent protein published in 2019, derived from Aequorea australis. It has low acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Dimer Aequorea australis 25.7 kDa -

FPbase ID: C2FJQ


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
504 510 170,000 0.97 164.9 4.4    


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AausFP1 Sequence



We were surprised to discover a second green-emitting FP in A. cf. australis, AausFP1, that shares only 53% amino acid identity with avGFP (see Fig. 4). AausFP1 is the brightest FP discovered to date, with a nearly perfect quantum yield (0.97) and a peak extinction coefficient of 170,000 M−1cm−1, making it nearly fivefold brighter than EGFP on a per-molecule basis. These already extraordinary properties are further bolstered by a low fluorescence pKa (4.4) and unusually narrow excitation and emission peaks (see Fig. 3; the emission peak of AausFP1 has a full width at half maximum (FWHM) of 19nm, compared to 32nm for EGFP).

Lambert et al. (2019)

Primary Reference

Additional References

  1. Live-cell nanoscopy with spontaneous blinking of conventional green fluorescent proteins

    Gavrikov As, Baranov Ms, Mishin As

    (2020). Biochemical and Biophysical Research Communications, 522(4) , 852-854. doi: 10.1016/j.bbrc.2019.11.163. Article   Pubmed

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