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Comparison List

PSmOrange

PSmOrange is a photoconvertible orange fluorescent protein published in 2011, derived from Discosoma sp.. It has high acid sensitivity.
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PSmOrange Spectrum Fluorescent protein PSmOrange excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.8 kDa -

FPbase ID: AQ8G3

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Far-red 634 662 32,700 0.28 9.16 5.6    
Orange 548 565 113,300 0.51 57.78 6.2 96.0  
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Transitions

From To Switch λ
Orange Far-red 480
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Photostability

No photostability measurements available ... add one!

PSmOrange Sequence

BLAST

PSmOrange was derived from mOrange with the following mutations: S21T/Q64L/F99Y/L124M/K162R/P186S
amino acid numbers relative to DsRed. show relative to mOrange

MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGFQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYEDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
GenBank: JN376081

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for PSmOrange
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A photoswitchable orange-to-far-red fluorescent protein, PSmOrange

Subach Om, Patterson Gh, Ting L-M, Wang Y, Condeelis Js, Verkhusha Vv

(2011). Nature Methods, 8(9) , 771-777. doi: 10.1038/nmeth.1664. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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