You can share this comparison at: www.fpbase.org/compare/egfp
Name | λex | λem | StokesλΔ | EC | QY | Brightness | pKa | Aggregation | Maturation | Lifetimeτ | kDa | Switch Type | Aliases |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
EGFP (EGFP) | 488 | 507 | 19 | 55,900 | 0.6 | 33.54 | 6.0 | wd | 25.0 | 2.6 | 26.94 | b | ['enhanced GFP', 'GFPmut1'] |
Sequence information not available for at least two proteins...
Cormack et al. (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene.Journal
Yang et al. (1996) Optimized Codon Usage and Chromophore Mutations Provide Enhanced Sensitivity with the Green Fluorescent Protein. Nucleic Acids Research.Journal
Zhang et al. (1996) An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells. Biochemical and Biophysical Research Communications.Journal Pubmed
Patterson et al. (1997) Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophysical Journal.Journal
Cubitt et al. (1998) Chapter 2: Understanding Structure—Function Relationships in the Aequorea victoria Green Fluorescent Protein. Methods in Cell Biology.Journal
Tsien (1998) THE GREEN FLUORESCENT PROTEIN. Annual Review of Biochemistry.Journal Pubmed
Heikal et al. (2001) Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP): acid–base specificity. Chemical Physics.Journal
Drobizhev et al. (2011) Two-photon absorption properties of fluorescent proteins. Nature Methods.Journal Pubmed
Arpino et al. (2012) Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222. PLoS ONE.Journal Pubmed
Sarkisyan et al. (2015) Green Fluorescent Protein with Anionic Tryptophan-Based Chromophore and Long Fluorescence Lifetime. Biophysical Journal.Journal Pubmed
Molina et al. (2017) Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation. The Journal of Physical Chemistry Letters.Journal Pubmed
Myšková et al. (2020) Directionality of light absorption and emission in representative fluorescent proteins. Proceedings of the National Academy of Sciences.Journal Pubmed