Osuga et al. (2021) :: Fluorescent Protein Database

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Development of a green reversibly photoswitchable variant of Eos fluorescent protein with fixation resistance

Osuga M, Nishimura T, Suetsugu S

(2021). Molecular Biology of the Cell, 32(21) , . doi: 10.1091/mbc.e21-01-0044. Article

Primary Proteins:

frSkylan-S

Add photostability measurements

Excerpts

We combined the amino acid substitutions of two Eos protein derivatives, Skylan-S and mEos4b, which are a green reversibly photoswitchable fluorescent protein (RSFP) and a fixation-resistant green-to-red photoconvertible fluorescent protein, respectively, resulting in the fixation-resistant Skylan-S (frSkylan-S), a green RSFP.