Excerpts

"Flamindo consists of an yellow FP variant, Citrine, fused with a cAMP binding domain of mEPAC1 (GenBank NP_001171281, 199–358 aa) in the vicinity of its chromophore. To improve the performance of Flamindo, we created many candidates by addition or deletion of amino acids in the N-terminus or C-terminus of the cAMP binding domain by PCR (Fig. S1). A mutant indicator with the largest dynamic range was named Flamindo2"