Comparison List


Flamindo2 is a green fluorescent protein published in 2014.

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Oligomerization Organism Molecular Weight Cofactor
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FPbase ID: J5AWO


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
504 523          


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Flamindo2 Sequence

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"Flamindo consists of an yellow FP variant, Citrine, fused with a cAMP binding domain of mEPAC1 (GenBank NP_001171281, 199–358 aa) in the vicinity of its chromophore. To improve the performance of Flamindo, we created many candidates by addition or deletion of amino acids in the N-terminus or C-terminus of the cAMP binding domain by PCR (Fig. S1). A mutant indicator with the largest dynamic range was named Flamindo2"

Odaka et al. (2014)

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