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| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 504 | 523 |
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"Flamindo consists of an yellow FP variant, Citrine, fused with a cAMP binding domain of mEPAC1 (GenBank NP_001171281, 199–358 aa) in the vicinity of its chromophore. To improve the performance of Flamindo, we created many candidates by addition or deletion of amino acids in the N-terminus or C-terminus of the cAMP binding domain by PCR (Fig. S1). A mutant indicator with the largest dynamic range was named Flamindo2"
Odaka et al. (2014)
(2014). PLoS ONE, 9(6) , e100252. doi: 10.1371/journal.pone.0100252. Article Pubmed
(2019). Communications Biology, 2(1) , 34. doi: 10.1038/s42003-018-0273-6. Article Pubmed
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