A bright and photostable photoconvertible fluorescent protein

Mckinney Sa, Murphy Cs, Hazelwood Kl, Davidson Mw, Looger Ll

(2009). Nature Methods, 6(2) , 131-133. doi: 10.1038/nmeth.1296. Article   Pubmed

    Primary Proteins:
  1. mEos2

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
mEos2 (Green) 42.0 175.0 mW/cm2 Arc-lamp Widefield H2B
mEos2 (Green) 240.0 100.0 µW Laser Point Scanning Confocal H2B
mEos2 (Red) 323.0 475.0 mW/cm2 Arc-lamp Widefield H2B
mEos2 (Red) 2700.0 100.0 µW Laser Point Scanning Confocal H2B
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

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