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Comparison List

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rsTagRFP

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Dec 20, 2018 03:46:57. Or view the full change history

rsTagRFP is a photoswitchable red fluorescent protein published in 2010, derived from Entacmaea quadricolor. It has high acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Entacmaea quadricolor	26.6 kDa	-

FPbase ID: 3FC3D

Attributes

State	Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
OFF			15,300	0.0013	0.02
ON	567	585	36,800	0.11	4.05	6.6

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Transitions

From	To	Switch λ
OFF	ON	440
ON	OFF	567

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Photostability

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rsTagRFP Sequence

BLAST

rsTagRFP was derived from TagRFP with the following mutations: S2_S2delinsVSKGE/F80W/I121L/N143A/S158G/F174L/K182N/Y194F/*232LextNS2_S2delinsVSKGE/F80W/I121L/N143A/S158G/F174L/K182N/Y194F/*230LextN
amino acid numbers relative to TagRFPeqFP578. show relative to eqFP578 show relative to TagRFP

MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEAATEMLYPADGGLEGRGDMALKLVGGGHLICNLKTTYRSKNPAKNLKMPGVYFVDHRLERIKEADKETYVEQHEVAVARYCDLPSKLGHKLN

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for rsTagRFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).