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Comparison List

rsKame

rsKame is a photoswitchable green fluorescent protein published in 2014, derived from Echinophyllia sp. SC22.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Echinophyllia sp. SC22 25.5 kDa -

FPbase ID: D87D7

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Off              
On 503 517 72,000 0.86 61.92      

Transitions

From To Switch λ
Off On 405
On Off 488

Photostability

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rsKame Sequence

rsKame was derived from Dronpa with the following mutations: V157L

MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVKEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNATNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDLNMALSLEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQAK

Excerpts

No excerpts have been added for rsKame
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Optimized two-color super resolution imaging of Drp1 during mitochondrial fission with a slow-switching Dronpa variant

Rosenbloom Ab, Lee S-H, To M, Lee A, Shin Jy, Bustamante C

(2014). Proceedings of the National Academy of Sciences, 111(36) , 13093-13098. doi: 10.1073/pnas.1320044111. Article   Pubmed

Additional References

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External Resources

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