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|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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RRvT was derived from tdTomato with the following mutations: K40cN/G42u_T42xdelinsYGMDELYK/T42h_V42rdelinsPMVSKGEEA/R42bS/K92R/L113I/M124V/I210V
amino acid numbers relative to DsRed. show relative to tdTomato
The brightest of the effectively monomeric DsRed variants is tdTomato—a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green-red heterodimeric (GRvT), tdTomato variants.