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Comparison List

RRvT

RRvT is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Discosoma sp.. It has very low acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
Tandem dimer Discosoma sp. 54.6 kDa -

FPbase ID: 7GO5D

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
556 583 134,000 0.88 117.92 3.9    

Photostability

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RRvT Sequence

RRvT was derived from tdTomato with the following mutations: K40cN/G42u_T42xdelinsYGMDELYK/T42h_V42rdelinsPMVSKGEEA/R42bS/K92R/L113I/M124V/I210V
amino acid numbers relative to DsRed. show relative to tdTomato

MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYNVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLYGMDELYKGSTGSGSSGPMVSKGEEAIKEFMRFKVSMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFRWERVMNFEDGGLVTVTQDSSIQDGTLIYKVKVRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTVVEQYERSEGRHHLFLYGMDELYK

Excerpts

The brightest of the effectively monomeric DsRed variants is tdTomato—a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green-red heterodimeric (GRvT), tdTomato variants.

Wiens et al. (2016)

Primary Reference

Additional References

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External Resources

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