compare

Comparison List

RRvT

RRvT is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Discosoma sp.. It has very low acid sensitivity.

No spectrum has been submitted ... add a spectrum!

Oligomerization Organism Molecular Weight Cofactor
Tandem dimer Discosoma sp. 54.6 kDa -

FPbase ID: 7GO5D

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
556 583 134,000 0.88 117.92 3.9    
Edit States/Attributes

Photostability

No photostability measurements available ... add one!

RRvT Sequence

BLAST

RRvT was derived from tdTomato with the following mutations: K40cN/G42u_T42xdelinsYGMDELYK/T42h_V42rdelinsPMVSKGEEA/R42bS/K92R/L113I/M124V/I210V
amino acid numbers relative to DsRed. show relative to tdTomato

MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYNVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLYGMDELYKGSTGSGSSGPMVSKGEEAIKEFMRFKVSMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFRWERVMNFEDGGLVTVTQDSSIQDGTLIYKVKVRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTVVEQYERSEGRHHLFLYGMDELYK

Excerpts

The brightest of the effectively monomeric DsRed variants is tdTomato—a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green-red heterodimeric (GRvT), tdTomato variants.

Wiens et al. (2016)

Primary Reference

A Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency

Wiens Md, Shen Y, Li X, Salem Ma, Smisdom N, Zhang W, Brown A, Campbell Re

(2016). ChemBioChem, 17(24) , 2361-2367. doi: 10.1002/cbic.201600492. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

Change history

Something missing or incorrect? Submit a change Submit a change