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Comparison List

GRvT

GRvT is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Discosoma sp.. It has very low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Tandem dimer Discosoma sp. 54.0 kDa -

FPbase ID: H93B4

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Red excitation 557 583 71,000 0.97 68.87 2.5    
FRET excitation 477 583 89,000 0.41 36.49 3.3    
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Photostability

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GRvT Sequence

BLAST

GRvT was derived from RRvT with the following mutations: Q40xL/F40yL/A40eM/E40bV/V40oA/N40cE/I42eR/G42vD/M42w_K42bdel/P42lT/A42tV/S42dR/R92K/V124M/V210I
amino acid numbers relative to DsRed. show relative to RRvT

MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPLLMYGSKMYVKHPADIPDYKKLSFPEGFKWVRVMNFEDGGLVTATQDSSLQDGTLIYEVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTRVEQYERSEGRHHLFLYDGSTGSGSSGTMVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSIQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLYGMDELYK

Excerpts

No excerpts have been added for GRvT
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency

Wiens Md, Shen Y, Li X, Salem Ma, Smisdom N, Zhang W, Brown A, Campbell Re

(2016). ChemBioChem, 17(24) , 2361-2367. doi: 10.1002/cbic.201600492. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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