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mKillerOrange

mKillerOrange is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2015, derived from Anthoathecata.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Anthoathecata	26.4 kDa	-

FPbase ID: QB9WJ

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
458	560	35,000	0.5	17.5

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Photostability

No photostability measurements available ... add one!

mKillerOrange Sequence

BLAST

mKillerOrange was derived from KillerOrange with the following mutations: C3V/S113D/L160T/F162T/L172K/L177F/M204T/H221Y/Q236E C3V/S113D/L160T/F162T/L172K/L177F/M204T/H221Y/Q232dE
amino acid numbers relative to KillerOrange anm2CP. show relative to anm2CP show relative to KillerOrange

MEVGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNVHAVCETGKLPMSWKPICHLIQWGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDGTMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQTATIGFTTADGGKMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQTRDTSDKRDHVCQREVAYAHSVPRITSAIGSDED

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for mKillerOrange
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore

Pletneva Nv, Pletnev Vz, Sarkisyan Ks, Gorbachev Da, Egorov Es, Mishin As, Lukyanov Ka, Dauter Z, Pletnev S

(2015). PLOS ONE, 10(12) , e0145740. doi: 10.1371/journal.pone.0145740. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for mKillerOrange
Search RIKEN BRC for mKillerOrange

Change history

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