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Comparison List

mHoneydew

mHoneydew is a basic (constitutively fluorescent) green fluorescent protein published in 2004, derived from Discosoma sp.. It has low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 25.4 kDa -

FPbase ID: FLO5T

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
487 562 17,000 0.12 2.04 4.0    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
5.9   Shaner et al. (2004)
A caution on interpretation of photostability measurements
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mHoneydew Sequence

BLAST

mHoneydew was derived from mRFP1.1 with the following mutations: Y67W/E144A/S146T/S147T/T195I/I197G

Note: Amino acid 147 is inconsistently reported: Fig 2 in Shaner et al. (2004) and patent WO2006024041A2 (Fig 48A) show 147S. However, the GenBank AAV52171, patent WO2006024041A2 (Fig 44), SnapGene, and Addgene 91760 sequences all have 147T. Here we use 147T which is likely to be found in most plasmids.

MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMWGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWAATTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKIDGKLDITSHNEDYTIVEQYERAEGRHSTGA
GenBank: AAV52171
IPG: 3838140

Excerpts

No excerpts have been added for mHoneydew
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry

(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037. Article   Pubmed

Additional References

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External Resources

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