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| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Lobophyllia hemprichii | 25.9 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| Green | 504 | 515 | 77,120 | 0.68 | 52.44 | 6.3 | 81.0 | |
| Red | 569 | 580 | 57,200 | 0.63 | 36.04 | 6.3 |
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mEos4b-L93M was derived from mEos4b with the following mutations: L93M
amino acid numbers relative to EosFP. show relative to mEos4b
Our measurements showed that, while the mutation V69A further slowed down the maturation kinetics of mEos4b, the three other single-point mutants improved it. Despite this, the V69T and I157V mutations were still unsatisfactory, with maturation time constants exceeding 400 min. In contrast, the L93M mutant showed a drastically decreased apparent maturation time of approximately 80 min, a more than 20-fold improvement relative to the mEos4b parent and better than anyother L93 substitution we have tested.
Maity et al. (2025)
(2025). Protein Science, 34(8) , e70234. doi: 10.1002/pro.70234. Article Pubmed
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