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Comparison List

GdT

a.k.a. dTG

GdT is a basic (constitutively fluorescent) green fluorescent protein published in 2016, derived from Discosoma sp.. It has very low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Discosoma sp. 27.0 kDa -

FPbase ID: HQZZC

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
476 500 59,000 0.14 8.26 3.8    
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Photostability

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GdT Sequence

BLAST

GdT was derived from dTomato with the following mutations: Q64L/F65L/A71M/E94V/V107A/K121E/I210R/G225bD
amino acid numbers relative to DsRed. show relative to dTomato

MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPLLMYGSKMYVKHPADIPDYKKLSFPEGFKWVRVMNFEDGGLVTATQDSSLQDGTLIYEVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTRVEQYERSEGRHHLFLYDMDELYK

Excerpts

When fused into a tandem dimer it is known as GGvT. Has also been fused with a variant red dtomato monomer to make GRvT creating a pseudo long stokes shift FP though FRET. Likely dimerizes with base dTomato.

Wiens et al. (2016)

Primary Reference

A Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency

Wiens Md, Shen Y, Li X, Salem Ma, Smisdom N, Zhang W, Brown A, Campbell Re

(2016). ChemBioChem, 17(24) , 2361-2367. doi: 10.1002/cbic.201600492. Article   Pubmed

Additional References

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