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Comparison List

DsRed.T3

DsRed.T3 is a basic (constitutively fluorescent) red fluorescent protein published in 2002, derived from Discosoma sp.. It is reported to be a slowly-maturing tetramer.

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Aggregation Organism Molecular Weight Cofactor
Tetramer Discosoma sp. 25.8 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
560 587 49,500 0.59 29.2   78.0  

Photostability

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DsRed.T3 Sequence

DsRed.T3 was derived from DsRed with the following mutations: R2A/K5E/N6D/T21S/H41T/N42Q/V44A/A145P

MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHHLFL

Excerpts

Compared with DsRed.T1 and DsRed.T4, DsRed.T3 is somewhat brighter but has a significantly higher peak of blue excitation and a marginally higher peak of green emission

Bevis & Glick (2002)

Primary Reference

Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)

Bevis Bj, Glick Bs

(2002). Nature Biotechnology, 20(1) , 83-87. doi: 10.1038/nbt0102-83. Article   Pubmed

Additional References

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External Resources

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