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Comparison List

CyOFP1

CyOFP1 is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2016, derived from Entacmaea quadricolor. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity.
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Aggregation Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.4 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
497 589 40,000 0.76 30.4 5.5 15.0 3.6

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
111.0 Chu et al. (2016)

CyOFP1 Sequence

CyOFP1 was derived from mNeptune2 with the following mutations: H10R/M11S/M15L/T18S/N21G/H23Q/S28H/T38K/G41N/C61H/T68_I76delinsVFIKYPADL/F79Y/T94M/T95V/Y96F/V104A/C114E/L121V/S128A/A142P/L147M/M160K/C172V/L174F/R179K/A184_N186del/L187V/Y193H/F194Y/H214Y/V216H/C222_N230cdelinsSNLGG
amino acid numbers relative to eqFP578. show relative to mNeptune2

MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTNRIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGGVLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCDKALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARYSNLGGGMDELYK

Excerpts

No excerpts have been added for CyOFP1
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

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