Comparison List


Citrine2 is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2018, derived from Aequorea victoria. It has moderate acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Dimer Aequorea victoria 26.9 kDa -

FPbase ID: ZWH88


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
509 522 98,000 0.7 68.6 5.9   3.3


t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
5.34 50.0 (mW) Laser Point Scanning Confocal
59.63 Arc-lamp Widefield

Citrine2 Sequence

Citrine2 was derived from mCitrine with the following mutations: S30T/M69T/Y145H/N149Y/V163A/K206Q/K214E/M218T/D234G
amino acid numbers relative to avGFP. show relative to mCitrine



Using this system, we developed Citrine2 with double the photostability, and equivalent brightness, compared to the starting template, mCitrine.

Wiens et al. (2018)

Primary Reference

Additional References

    No additional references have been added.

External Resources

Change history

Something missing or incorrect? Submit a change Submit a change