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Comparison List

BFP

a.k.a. P4-3

BFP is a basic (constitutively fluorescent) blue fluorescent protein published in 1996, derived from Aequorea victoria.

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Oligomerization Organism Molecular Weight Cofactor
Dimer Aequorea victoria 26.8 kDa -

FPbase ID: SDS4K

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
381 445 14,000 0.38 5.32      
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Photostability

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BFP Sequence

BLAST

BFP was derived from P4 with the following mutations: Y145F

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Excerpts

No excerpts have been added for BFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

Heim R, Tsien Ry

(1996). Current Biology, 6(2) , 178-182. doi: 10.1016/s0960-9822(02)00450-5. Article

Additional References

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External Resources

Change history

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