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Comparison List

mIFP

mIFP is a basic (constitutively fluorescent) near ir fluorescent protein published in 2015, derived from Bradyrhizobium sp.. It has very low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Bradyrhizobium sp. 35.1 kDa Biliverdin

FPbase ID: LDVKW

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
683 704 82,000 0.08 6.56 3.5    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
54.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)
90.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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mIFP Sequence

BLAST
MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVVIQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVDCTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYDRVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYTPVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIACHHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK

Excerpts

No excerpts have been added for mIFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A naturally monomeric infrared fluorescent protein for protein labeling in vivo

Yu D, Baird Ma, Allen Jr, Howe Es, Klassen Mp, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang S-Q, Weiner Od, Kornberg Tb, Jan Y-N, Davidson Mw, Shu X

(2015). Nature Methods, 12(8) , 763-765. doi: 10.1038/nmeth.3447. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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