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Comparison List

eqFP650

a.k.a. TurboFP650

eqFP650 is a basic (constitutively fluorescent) red fluorescent protein published in 2010, derived from Entacmaea quadricolor. It has moderate acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Entacmaea quadricolor 26.2 kDa -

FPbase ID: JS838

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
592 650 65,000 0.24 15.6 5.7    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
404.0 Widefield 25.0 Luker et al. (2015)
A caution on interpretation of photostability measurements
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eqFP650 Sequence

BLAST

eqFP650 was derived from Katushka-9-5 with the following mutations: D21G/M41A
amino acid numbers relative to eqFP578. show relative to Katushka-9-5

MGEDSELISENMHMKLYMEGTVNGHHFKCTSEGEGKPYEGTQTAKIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQMALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRKLERIKEADKETYVEQHEMAVARYCDLPSKLGHS
GenBank: ADR83529

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for eqFP650
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Near-infrared fluorescent proteins

Shcherbo D, Shemiakina Ii, Ryabova Av, Luker Ke, Schmidt Bt, Souslova Ea, Gorodnicheva Tv, Strukova L, Shidlovskiy Km, Britanova Ov, Zaraisky Ag, Lukyanov Ka, Loschenov Vb, Luker Gd, Chudakov Dm

(2010). Nature Methods, 7(10) , 827-829. doi: 10.1038/nmeth.1501. Article   Pubmed

Additional References

  1. Two-photon absorption properties of fluorescent proteins

    Drobizhev M, Makarov Ns, Tillo Se, Hughes Te, Rebane A

    (2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article   Pubmed

External Resources

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