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Comparison List

P4

a.k.a. Y66H

P4 is a basic (constitutively fluorescent) blue fluorescent protein published in 1994, derived from Aequorea victoria.
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Oligomerization Organism Molecular Weight Cofactor
Weak dimer Aequorea victoria 26.9 kDa -

FPbase ID: 671NA

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
382 448 2,100 0.24 0.5      
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Photostability

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P4 Sequence

BLAST

P4 was derived from avGFP with the following mutations: Y66H

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Excerpts

No excerpts have been added for P4
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Wavelength mutations and posttranslational autoxidation of green fluorescent protein.

Heim R, Prasher Dc, Tsien Ry

(1994). Proceedings of the National Academy of Sciences, 91(26) , 12501-12504. doi: 10.1073/pnas.91.26.12501. Article

Additional References

  1. Chapter 2: Understanding Structure—Function Relationships in the Aequorea victoria Green Fluorescent Protein

    Cubitt Ab, Woollenweber La, Heim R

    (1998). Methods in Cell Biology, , 19-30. doi: 10.1016/s0091-679x(08)61946-9. Article

  2. THE GREEN FLUORESCENT PROTEIN

    Tsien Ry

    (1998). Annual Review of Biochemistry, 67(1) , 509-544. doi: 10.1146/annurev.biochem.67.1.509. Article   Pubmed

  3. Crystal Structure and Photodynamic Behavior of the Blue Emission Variant Y66H/Y145F of Green Fluorescent Protein†

    Wachter Rm, King Ba, Heim R, Kallio K, Tsien Ry, Boxer Sg, Remington Sj

    (1997). Biochemistry, 36(32) , 9759-9765. doi: 10.1021/bi970563w. Article   Pubmed

External Resources

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