Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells

Erdogan M, Fabritius A, Basquin J, Griesbeck O

(2020). Cell Chemical Biology, , . doi: 10.1016/j.chembiol.2020.02.004. Article   Pubmed

    Primary Proteins:
  1. mCRISPRed
Add photostability measurements

Excerpts

pH titration showed that mCRISPRed had the lowest pKa value (pKa = 2.1) among all fluorescent proteins known to date, surpassing that of TagBFP2 (pKa = 2.7), those of other comparable long Stokes shift red fluorescent proteins, and that of a green fluorescent protein variant named Gamillus engineered for increased pH resistance (pKa = 3.4). These properties render it very favorable for applications in acidic environments, such as lysosomes.