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vsfGFP-9

a.k.a. vGFP, vsfGFP

vsfGFP-9 is a basic (constitutively fluorescent) green fluorescent protein published in 2015, derived from Aequorea victoria. It has low acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Aequorea victoria	39.5 kDa	-

FPbase ID: ACXOE

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
485	510	104,958	0.7	73.47	4.75

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Photostability

No photostability measurements available ... add one!

vsfGFP-9 Sequence

BLAST

vsfGFP-9 was derived from Superfolder GFP with the following mutations: *239AextQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTV*237AextQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTV
amino acid numbers relative to Superfolder GFPavGFP. show relative to avGFP show relative to Superfolder GFP

MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKAQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVS

Excerpts

No excerpts have been added for vsfGFP-9
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).