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Comparison List

ShyRFP

ShyRFP is a multi-photochromic long stokes shift fluorescent protein published in 2014, derived from Discosoma sp..

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Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.9 kDa -

FPbase ID: 1M59L

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Yellow state 450 596 30,000          
Red state 545   142,000          

Transitions

From To Switch λ
Yellow state Red state 405

Photostability

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ShyRFP Sequence

ShyRFP was derived from mApple with the following mutations: W143A/S146T/I161S/K163E/R164W
amino acid numbers relative to DsRed. show relative to mApple

MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGLEATEERMYPEDGALKSESKEWLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAEGRHSTGGMDELYK

Excerpts

The purified protein appeared yellow due to the blue light absorbing neutral chromophore. However, when this protein solution was left on ice for a few minutes under ambient light, it was observed to turn a bright magenta colour.

Shen et al. (2014)

Primary Reference

A photochromic and thermochromic fluorescent protein

Shen Y, Wiens Md, Campbell Re

(2014). RSC Adv., 4(100) , 56762-56765. doi: 10.1039/c4ra10107c. Article

Additional References

    No additional references have been added.

External Resources

Change history

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