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ShyRFP

This protein has unmoderated changes.

ShyRFP is a multi-photochromic long stokes shift fluorescent protein published in 2014, derived from Discosoma sp..

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Discosoma sp.	26.9 kDa	-

FPbase ID: 1M59L

Attributes

State	Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
Yellow state	450	596	30,000
Red state	545		142,000

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Transitions

From	To	Switch λ
Yellow state	Red state	405

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Photostability

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ShyRFP Sequence

ShyRFP was derived from mApple with the following mutations: W148A/S151T/I166S/K168E/R169WW143A/S146T/I161S/K163E/R164W
amino acid numbers relative to mAppleDsRed. show relative to DsRed show relative to mApple

MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGLEATEERMYPEDGALKSESKEWLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAEGRHSTGGMDELYK

Excerpts

The purified protein appeared yellow due to the blue light absorbing neutral chromophore. However, when this protein solution was left on ice for a few minutes under ambient light, it was observed to turn a bright magenta colour.

Shen et al. (2014)

Primary Reference

A photochromic and thermochromic fluorescent protein

Shen Y, Wiens Md, Campbell Re

(2014). RSC Adv., 4(100) , 56762-56765. doi: 10.1039/c4ra10107c. Article

Additional References

No additional references have been added.

External Resources

Search Addgene for ShyRFP

Change history

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