This protein has unmoderated changes.
ShyRFP is a multi-photochromic long stokes shift fluorescent protein published in 2014, derived from Discosoma sp..
No photostability measurements available ...
ShyRFP was derived from
mApple with the following mutations: W143A/S146T/I161S/K163E/R164W
amino acid numbers relative to
DsRed. show relative to mApple
The purified protein appeared yellow due to the blue light absorbing neutral chromophore. However, when this protein solution was left on ice for a few minutes under ambient light, it was observed to turn a bright magenta colour.
Shen et al. (2014)
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RSC Adv., 4(100) , 56762-56765. doi: 10.1039/c4ra10107c.
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