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Comparison List

ShadowG

ShadowG is a basic (constitutively fluorescent) green fluorescent protein published in 2015, derived from Aequorea victoria.
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ShadowG Spectrum Fluorescent protein ShadowG excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
? Aequorea victoria 27.0 kDa -

FPbase ID: JM7JP

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
486 510 89,000 0.005 0.45     0.16
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Photostability

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ShadowG Sequence

BLAST

ShadowG was derived from Nữ with the following mutations: V148H/Y203T/A206K/R223F
amino acid numbers relative to avGFP. show relative to Nữ

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

Excerpts

No excerpts have been added for ShadowG
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer

Murakoshi H, Shibata Ace, Nakahata Y, Nabekura J

(2015). Scientific Reports, 5(1) , . doi: 10.1038/srep15334. Article

Additional References

    No additional references have been added.

External Resources

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