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Comparison List

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PSmOrange2

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Dec 17, 2018 00:52:34. Or view the full change history

PSmOrange2 is a photoconvertible orange fluorescent protein published in 2012, derived from Discosoma sp.. It has high acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Discosoma sp.	26.7 kDa	-

FPbase ID: U76BO

Attributes

State	Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
Orange	546	561	51,000	0.61	31.11	6.6	210.0
Far-red	619	651	18,900	0.38	7.18	5.4

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Transitions

From	To	Switch λ
Orange	Far-red	489

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Photostability

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PSmOrange2 Sequence

BLAST

PSmOrange2 was derived from PSmOrange with the following mutations: R22H/R41H/F70I/Q193L/A222S/G224AR17H/R36H/F65I/Q188L/A217S/G219A
amino acid numbers relative to PSmOrangeDsRed. show relative to DsRed show relative to PSmOrange

MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEGFQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYEDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERSEARHSTGGMDELYK

GenBank: AFK91500

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for PSmOrange2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A FRET-Facilitated Photoswitching Using an Orange Fluorescent Protein with the Fast Photoconversion Kinetics

Subach Om, Entenberg D, Condeelis Js, Verkhusha Vv

(2012). Journal of the American Chemical Society, 134(36) , 14789-14799. doi: 10.1021/ja3034137. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for PSmOrange2
Search RIKEN BRC for PSmOrange2

Change history

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