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Comparison List

PSmOrange2

PSmOrange2 is a photoconvertible orange fluorescent protein published in 2012, derived from Discosoma sp.. It has high acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.7 kDa -

FPbase ID: U76BO

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Orange 546 561 51,000 0.61 31.11 6.6 210.0  
Far-red 619 651 18,900 0.38 7.18 5.4    

Transitions

From To Switch λ
Orange Far-red 489

Photostability

No photostability measurements available ... add one!

PSmOrange2 Sequence

PSmOrange2 was derived from PSmOrange with the following mutations: R17H/R36H/F65I/Q188L/A217S/G219A
amino acid numbers relative to DsRed. show relative to PSmOrange

MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEGFQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYEDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERSEARHSTGGMDELYK
GenBank: AFK91500

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for PSmOrange2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A FRET-Facilitated Photoswitching Using an Orange Fluorescent Protein with the Fast Photoconversion Kinetics

Subach Om, Entenberg D, Condeelis Js, Verkhusha Vv

(2012). Journal of the American Chemical Society, 134(36) , 14789-14799. doi: 10.1021/ja3034137. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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