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Comparison List

mRouge

mRouge is a basic (constitutively fluorescent) far red fluorescent protein published in 2010, derived from Discosoma sp.. It has high acid sensitivity.
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mRouge Spectrum Fluorescent protein mRouge excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.8 kDa -

FPbase ID: JXVU8

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
600 637 43,000 0.02 0.86 6.1    
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Photostability

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mRouge Sequence

BLAST

mRouge was derived from mCherry with the following mutations: V16T/S146C/I161M/Q163M/V195T/I197T/A217N
amino acid numbers relative to DsRed. show relative to mCherry

MVSKGEEDNMAIIKEFMRFKTHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEACSERMYPEDGALKGEMKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNTNTKLDITSHNEDYTIVEQYERNEGRHSTGGMDELYK

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for mRouge
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Generation of longer emission wavelength red fluorescent proteins using computationally designed libraries

Chica Ra, Moore Mm, Allen Bd, Mayo Sl

(2010). Proceedings of the National Academy of Sciences, 107(47) , 20257-20262. doi: 10.1073/pnas.1013910107. Article   Pubmed

Additional References

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External Resources

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