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Comparison List

miRFP2

miRFP2 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2021, derived from Rhodopseudomonas palustris. It has very low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.3 kDa Biliverdin

FPbase ID: AYRSR

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
676 706 55,600 0.043 2.39 3.7    
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Photostability

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miRFP2 Sequence

BLAST

miRFP2 was derived from miRFP with the following mutations: D16S/V66I/P85L/P127S/A155V/Q175R/E211V/S222T/E300V

MVAGHASGSPDFGTASPSDCEHEEIHLAGSIQPHGTLLVVSEPDHRIIQASANAAEFLNLGSVLGIPLAEIDGDLLIKILPHLDLTAEGMPVAVRCRIGNPSTEYDGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDERGHGEVYSEIHVTGLESYFGNRYPSSLVPQMARRLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPTHLQFLKNMGVRATLVVSLVVGGKLWGLVICHHYLPRFIHFELRAICVLLAEAIATRITAL
GenBank: MZ682639

Excerpts

No excerpts have been added for miRFP2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells

Babakhanova S, Jung Ee, Namikawa K, Zhang H, Wang Y, Subach Om, Korzhenevskiy Da, Rakitina Tv, Xiao X, Wang W, Shi J, Drobizhev M, Park D, Eisenhard L, Tang H, Köster Rw, Subach Fv, Boyden Es, Piatkevich Kd

(2021). , , . doi: 10.1101/2021.08.02.454744. Article

Additional References

    No additional references have been added.

External Resources

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