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miRFP

miRFP is a basic (constitutively fluorescent) near ir fluorescent protein published in 2018, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.3 kDa Biliverdin

FPbase ID: HO9GG

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
674 703 92,400 0.097 8.96 4.3    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
432.0 LED Widefield 25.0 Piatkevich et al. (2018)
A caution on interpretation of photostability measurements
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miRFP Sequence

BLAST

miRFP was derived from RpBphP1 with the following mutations: A11D/L17P/N19D/A36T/F181Y/R184I/P187T/D201L/I202V/I253T/Y257F/A282I/E314_S315del

MVAGHASGSPDFGTADPSDCEREEIHLAGSIQPHGTLLVVSEPDHRIIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYDGLMHRPPEGGLIIELERAGPPIDLSGTLAPALERIRTAGSLRALCDDTALLFQQCTGYDRVMVYRFDEQGHGEVYSEIHVTGLESYFGNRYPSSLVPQMARRLYERQRVRVLVDVSYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPTHLQFLKNMGVRATLVVSLVVGGKLWGLVICHHYLPRFIHFELRAICELLAEAIATRITAL
GenBank: MG250278.1

Excerpts

No excerpts have been added for miRFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

Piatkevich Kd, Jung Ee, Straub C, Linghu C, Park D, Suk H-J, Hochbaum Dr, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang C-T, Rhoades Jl, Shemesh O, Asano S, Yoon Y-G, Freifeld L, Saulnier Jl, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens Mb, Flavell Sw, Sabatini Bl, Boyden Es

(2018). Nature Chemical Biology, , . doi: 10.1038/s41589-018-0004-9. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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