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Comparison List

mEos3.2

mEos3.2 is a photoconvertible green/yellow fluorescent protein published in 2012. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer <add one> 25.7 kDa -

FPbase ID: VUXRF

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Red 572 580 32,200 0.55 17.71 5.8    
Green 507 516 63,400 0.7 44.38 5.4 20.0  
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Transitions

From To Switch λ
Green Red 405
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Photostability

No photostability measurements available ... add one!

mEos3.2 Sequence

BLAST
MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKEGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNARNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLLEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNARR

Excerpts

No excerpts have been added for mEos3.2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Rational design of true monomeric and bright photoactivatable fluorescent proteins

Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, Liu Y, Zhang J, Xu P, Xu T

(2012). Nature Methods, 9(7) , 727-729. doi: 10.1038/nmeth.2021. Article   Pubmed

Additional References

  1. Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate

    Durisic N, Laparra-Cuervo L, Sandoval-Álvarez , Borbely Js, Lakadamyali M

    (2014). Nature Methods, 11(2) , 156-162. doi: 10.1038/nmeth.2784. Article   Pubmed

External Resources

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