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Comparison List

LSSmCherry1

LSSmCherry1 is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2017, derived from Discosoma sp.. It has high acid sensitivity.
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LSSmCherry1 Spectrum Fluorescent protein LSSmCherry1 excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.7 kDa -

FPbase ID: EY2GX

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
450 610 35,000 0.29 10.15 6.2    
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Photostability

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LSSmCherry1 Sequence

BLAST

LSSmCherry1 was derived from mCherry with the following mutations: A6bT/K92N/K138C/K139R/W143L/S147T/I161S/Q163E/N196D/T202L/A217S
amino acid numbers relative to DsRed. show relative to mCherry

MVSKGEEDNMTIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGLEASTERMYPEDGALKGESKERLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERSEGRHSTGGMDELYK

Excerpts

No excerpts have been added for LSSmCherry1
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Shen Y, Chen Y, Wu J, Shaner Nc, Campbell Re

(2017). PLOS ONE, 12(2) , e0171257. doi: 10.1371/journal.pone.0171257. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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